R01 [GM041935] and R35 [“type”:”entrez-nucleotide”,”attrs”:”text”:”GM122576″,”term_id”:”221982209″,”term_text”:”GM122576″GM122576]

R01 [GM041935] and R35 [“type”:”entrez-nucleotide”,”attrs”:”text”:”GM122576″,”term_id”:”221982209″,”term_text”:”GM122576″GM122576]. https://doi.org/10.1124/dmd.118.085670. This article has supplemental material available at dmd.aspetjournals.org.. 3.63 (brm, 1H), 0.97 (d, 3H, = 6 Hz), 0.89 (s, 3H), 0.66 (s, 3H); and for 13C-NMR (101 MHz, CDCl3) 174.8, 72.9, 71.7, 68.0, 51.5, 48.3, 47.6, 47.1, 46.5, 35.5, 35.5, 35.1, 34.9, 34.4, 32.8, 31.1, 30.8, 29.7, 28.7, 28.4, 27.4, 23.6, 23.1, 17.2, and 12.6. The following are the spectra data for the methyl 34.00 (m, 1H), 3.77 (m, 1H), 3.66 (s, 3H), 3.61 (brm, 1H), 1.09 (s, 3H), 0.97 (d, 3H, = 6 Hz), 0.71 (s, 3H); and for 13C-NMR (151 MHz, CDCl3) 174.7, 73.0, 72.9, 71.1, 51.5, 48.4, 47.8, 47.2, 46.4, 36.2, 35.6, 35.1, 34.2, 33.8, 33.8, 31.0, 30.8, 29.86, 28.3, 27.4, 25.2, 23.6, 17.2, and 12.7. Synthesis of DCA-55.09 (m, 1H), 5.05 (brm, 1H), 3.66 (s, 3H), 0.88 (s, 3H), 0.81 (d, 3H, = 6 Hz), and 0.73 (s, 3H). Synthesis of DCA-15.04 (m, 1H), 4.09 (brm, 1H), 3.83 (m, 1H), 3.66 (s, 3H), 2.08 (s, 3H), 1.03 (s, 3H), and 0.73 (s, 3H). Synthesis of DCA-25.09 (m, 1H), 3.66 (s, 3H), 3.43 (brm, 1H), 3.35 (brm, 1H), 0.94 (s, 3H), 0.79 (d, 3H, = 6 Hz), and 0.72 (s, 3H); 13C-NMR (151 MHz, CDCl3) 174.6, 170.6, 76.5, 75.8, 71.3, 51.5, 49.1, 47.5, 44.9, 43.1, 41.8, 36.7, 35.9, 35.7, 34.6, 33.6, 30.9, 30.7, 27.3, 26.3, 25.9, 25.8, 23.4, 23.0, 21.4, 17.5, and 12.3. The following are the spectra data for the methyl 35.05 (m, 1H), 3.72 (dd, 1H, = 9 Hz, 10 Hz), 3.66 (s, 3H), 3.39 (brm, 1H), 0.93 (s, 3H), 0.79 (d, 3H, = 6 Hz), and 0.72 (s, 3H); 13C-NMR (151 MHz, CDCl3) 174.6, 170.5, 76.5, 75.7, 72.4, 51.5, 49.4, 48.4, 47.5, 44.9, 36.4, 36.2, 35.5, 34.6, 34.1, 30.9, 30.7, 27.2, 27.1, 25.6, 25.5, 23.3, 23.2, 21.3, 20.7, 17.4, and 12.3. Human Serum and Urine. Postprandial human serum and urine were collected from 13 healthy adult volunteers (Ferslew et al., 2015). After ingestion of the standardized high-fat breakfast, urine samples were collected and pooled over the 2-hour period; blood samples were collected in untreated glass BMS-066 tubes at 0.0, 0.5, 1.0, 1.5, and 2.0 hours and allowed to clot for 30C60 minutes to separate the serum. This study was approved by the University of North Carolina at Chapel Hill (UNC-CH) Biomedical Institutional Review Board and published in ClinicalTrials.gov (“type”:”clinical-trial”,”attrs”:”text”:”NCT01766960″,”term_id”:”NCT01766960″NCT01766960). Overnight fasting spot urine samples were collected at West China Hospital of Sichuan University from 45 healthy volunteers including 30 men and 15 women (18C40 years old, body mass index 19C26). Briefly, the inclusion criteria for healthy subjects were normal blood, liver and kidney functions; negative test results for the biomarker of infectious diseases including hepatitis B, hepatitis C, HIV and Treponema pallidum; no abnormalities in electrocardiogram, abdominal ultrasonography and chest radiography; no history of gastrointestinal surgery except for appendicectomy; and no ingestion of any medications or dietary supplements 2 weeks before urine collections. The studies were approved by the Institutional Review Board of West China Hospital of Sichuan University. All serum and urine samples were stored at ?80C until analysis. Sample Preparation for BAs Analysis. Analysis of BAs metabolome were performed using the enzyme digestion techniques published in our recent work (Zhu et al., 2018). For the postprandial human serum and urine samples from 13 healthy adults, aliquot (50 for 20 minutes. Two hundred microliters of supernatant was vacuum-evaporated at 30C. The residue was reconstituted with 50 100C500 at a resolution of.For incubations using unconjugated BAs as substrates, the supernatant (50 0.75), which strengthened the hypothesis that TBA05, TBA09, TBA10 and TBA13 are hydroxylated metabolites of DCA. 3.83 (m, 1H), 4.31 (d, 1H, = 11 Hz), 3.94 (d, 1H, = 11 Hz), 3.66 (s, 3H), 0.82 (d, 3H, = 6 Hz), 0.73 (s, 3H). Synthesis of DCA-64.05 (brm, 1H), 3.99 (m, 1H), 3.66 (s, 3H), 3.63 (brm, 1H), 0.97 (d, 3H, = 6 Hz), 0.89 (s, 3H), 0.66 (s, 3H); and for 13C-NMR (101 MHz, CDCl3) 174.8, 72.9, 71.7, 68.0, 51.5, 48.3, 47.6, 47.1, 46.5, 35.5, 35.5, 35.1, 34.9, 34.4, 32.8, 31.1, 30.8, 29.7, 28.7, 28.4, 27.4, 23.6, 23.1, 17.2, and 12.6. The following are the spectra data for the methyl 34.00 (m, 1H), 3.77 (m, 1H), 3.66 (s, 3H), 3.61 (brm, 1H), 1.09 (s, 3H), 0.97 (d, 3H, = 6 Hz), 0.71 (s, 3H); and for 13C-NMR Dicer1 (151 MHz, CDCl3) 174.7, 73.0, 72.9, 71.1, 51.5, 48.4, 47.8, 47.2, 46.4, 36.2, 35.6, 35.1, 34.2, 33.8, 33.8, 31.0, 30.8, 29.86, 28.3, 27.4, 25.2, 23.6, 17.2, and 12.7. Synthesis of DCA-55.09 (m, 1H), 5.05 (brm, 1H), 3.66 (s, 3H), 0.88 (s, 3H), 0.81 (d, 3H, = 6 Hz), and 0.73 (s, 3H). Synthesis of DCA-15.04 (m, 1H), 4.09 (brm, 1H), 3.83 (m, 1H), 3.66 (s, 3H), 2.08 (s, 3H), 1.03 (s, 3H), and 0.73 (s, 3H). Synthesis of DCA-25.09 (m, 1H), 3.66 (s, 3H), 3.43 (brm, 1H), 3.35 (brm, 1H), 0.94 (s, 3H), 0.79 (d, 3H, = 6 Hz), and 0.72 (s, 3H); 13C-NMR (151 MHz, CDCl3) 174.6, 170.6, 76.5, 75.8, 71.3, 51.5, 49.1, 47.5, 44.9, 43.1, 41.8, 36.7, 35.9, 35.7, 34.6, 33.6, 30.9, 30.7, 27.3, 26.3, 25.9, 25.8, 23.4, 23.0, 21.4, 17.5, and 12.3. The following are the spectra data for the methyl 35.05 (m, 1H), 3.72 (dd, 1H, = 9 Hz, 10 Hz), 3.66 (s, 3H), 3.39 (brm, 1H), 0.93 (s, 3H), 0.79 (d, 3H, = 6 Hz), and 0.72 (s, 3H); 13C-NMR (151 MHz, CDCl3) 174.6, 170.5, 76.5, 75.7, 72.4, 51.5, 49.4, 48.4, 47.5, 44.9, 36.4, 36.2, 35.5, 34.6, 34.1, 30.9, 30.7, 27.2, 27.1, 25.6, 25.5, 23.3, 23.2, 21.3, 20.7, 17.4, and 12.3. Human Serum and Urine. Postprandial human serum and urine were collected from 13 healthy adult volunteers (Ferslew et al., 2015). After ingestion of the standardized high-fat breakfast, urine samples were collected and pooled over the 2-hour period; blood samples were collected in untreated glass tubes at 0.0, 0.5, 1.0, 1.5, and 2.0 hours and allowed to clot for 30C60 minutes to separate the serum. This study was approved by the University of North Carolina at Chapel Hill (UNC-CH) Biomedical Institutional Review Board and published in ClinicalTrials.gov (“type”:”clinical-trial”,”attrs”:”text”:”NCT01766960″,”term_id”:”NCT01766960″NCT01766960). Overnight fasting spot urine samples were collected at West China Hospital of Sichuan University from 45 healthy volunteers including 30 men and 15 women (18C40 years old, body mass index 19C26). Briefly, the inclusion criteria for healthy subjects were normal blood, liver and kidney functions; negative test BMS-066 BMS-066 results for the biomarker of infectious diseases including hepatitis B, hepatitis C, HIV and Treponema pallidum; no abnormalities in electrocardiogram, abdominal ultrasonography and chest radiography; no history of gastrointestinal surgery except for appendicectomy; and no ingestion of any medicines or health supplements 14 days before urine series. The studies had been accepted BMS-066 by the Institutional Review Plank of Western world China Medical center of Sichuan School. All serum and urine examples were kept at ?80C until evaluation. Sample Planning for BAs Evaluation. Evaluation of BAs metabolome had been performed using the enzyme digestive function techniques published inside our latest function (Zhu et al., 2018). For the postprandial individual serum and urine examples from 13 healthful adults,.