1997). RTCPCR The manufacturer’s protocols were used to extract RNA using Trizol Reagent (GIBCO-BRL), first-strand cDNA synthesis with oligo(dT) or random hexamer primers using the SuperScript First-Strand Synthesis System (Invitrogen), and PCR with Expand High-Fidelity polymerase (Roche). the inhibition of neurogenesis by (Supplemental Fig. 1B). The gene found in our […]
Daily Archives: October 20, 2024
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On the other hand, CS didn’t significantly affect the expression of multiple genes constituting phase I detoxification pathway, and Cyp1A1 was expressed in the emphysematous lungs of A/J mice predominantly. mice subjected to CS for 5 h, 8 times, and 1.5 and 6 mo, respectively. A lot of the genes […]