Briefly, bone marrow cells from femurs and tibia of 8C12-week-old mice were plated at 2105 cells/ml and cultured at 37C and 5% CO2 atmosphere, in complete RPMI (RPMI 1640 containing 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, 10 mM HEPES, 100 devices/ml penicillin G, 100 g/ml streptomycin, and 0.05 […]
Daily Archives: February 21, 2025
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